Error Correction and DeNovo Genome Assembly for the MinION Sequencing Reads mixing Illumina Short Re

Error Correction and DeNovo Genome Assembly for the MinION Sequencing Reads mixing Illumina Short Reads

混合Illumina短read和MinION测序read的错误纠正和从头基因组组装

抽象——

牛津纳米孔技术公司(Oxford Nanopore Technologies)提供的新型迷你音序器体积小，由一台笔记本电脑的USB 3.0接口供电。

该测序产生长读与低生产成本和高通量。

然而，由MinIon测序器产生的长读有很高的错误率(约25%[1])，这将恶化通过分析这些长读得到的结果的质量。

纠正长读的一个解决方案是利用第二代测序技术产生的高覆盖率和高质量的短读。在这里，我们提出了MiRCA (MlnIon读校正算法)，这是一种混合的管道，它可以检测和纠正MinIon长读的错误，使用预装配的IIIumina MiSeq短读，我们使用OverlapLayout- Consensus(OLC)方法来装配校正后的读。MiRCA能够纠正:删除、插入和替换错误，通过形成多个序列对齐，不需要很大的内存空间。我们使用酿酒酵母W303基因组和大肠杆菌K-12 MG1655细菌基因组来测试我们的管道的效率

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