【细胞分割】基于matlab GUI分水岭算法细胞分割计数【含Matlab源码 637期】

一、图像分割简介

理论知识参考：【基础教程】基于matlab图像处理图像分割【含Matlab源码 191期】

二、部分源代码

function varargout = CellCount(varargin)
% CELLCOUNT M-file for CellCount.fig
%      CELLCOUNT, by itself, creates a new CELLCOUNT or raises the existing
%      singleton*.
%
%      H = CELLCOUNT returns the handl e to a new CELLCOUNT or the handle to
%      the existing singleton*.
%
%      CELLCOUNT('CALLBACK',hObject,eventData,handles,...) calls the local
%      function named CALLBACK in CELLCOUNT.M with the given input arguments.
%
%      CELLCOUNT('Property','Value',...) creates a new CELLCOUNT or raises the
%      existing singleton*.  Starting from the left, property value pairs are
%      applied to the GUI before CellCount_OpeningFcn gets called.  An
%      unrecognized property name or invalid value makes property application
%      stop.  All inputs are passed to CellCount_OpeningFcn via varargin.
%
%      *See GUI Options on GUIDE's Tools menu.  Choose "GUI allows only one
%      instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES% Edit the above text to modify the response to help CellCount% Last Modified by GUIDE v2.5 01-May-2015 19:43:57% Begin initialization code - DO NOT EDIT
gui_Singleton = 1;
gui_State = struct('gui_Name',       mfilename, ...'gui_Singleton',  gui_Singleton, ...'gui_OpeningFcn', @CellCount_OpeningFcn, ...'gui_OutputFcn',  @CellCount_OutputFcn, ...'gui_LayoutFcn',  [] , ...'gui_Callback',   []);
if nargin && ischar(varargin{1})gui_State.gui_Callback = str2func(varargin{1});
endif nargout[varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
elsegui_mainfcn(gui_State, varargin{:});
end
% End initialization code - DO NOT EDIT% --- Executes just before CellCount is made visible.
function CellCount_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
% varargin   command line arguments to CellCount (see VARARGIN)% Choose default command line output for CellCount
handles.output = hObject;% Update handles structure
guidata(hObject, handles);% UIWAIT makes CellCount wait for user response (see UIRESUME)
% uiwait(handles.figure1);% --- Outputs from this function are returned to the command line.
function varargout = CellCount_OutputFcn(hObject, eventdata, handles)
% varargout  cell array for returning output args (see VARARGOUT);
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% Get default command line output from handles structure
varargout{1} = handles.output;function edit1_Callback(hObject, eventdata, handles)
% hObject    handle to edit1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% Hints: get(hObject,'String') returns contents of edit1 as text
%        str2double(get(hObject,'String')) returns contents of edit1 as a double% --- Executes during object creation, after setting all properties.
function edit1_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))set(hObject,'BackgroundColor','white');
endfunction edit3_Callback(hObject, eventdata, handles)
% hObject    handle to edit3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% Hints: get(hObject,'String') returns contents of edit3 as text
%        str2double(get(hObject,'String')) returns contents of edit3 as a double% --- Executes during object creation, after setting all properties.
function edit3_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))set(hObject,'BackgroundColor','white');
endfunction edit4_Callback(hObject, eventdata, handles)
% hObject    handle to edit4 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% Hints: get(hObject,'String') returns contents of edit4 as text
%        str2double(get(hObject,'String')) returns contents of edit4 as a double% --- Executes during object creation, after setting all properties.
function edit4_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit4 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))set(hObject,'BackgroundColor','white');
endfunction edit5_Callback(hObject, eventdata, handles)
% hObject    handle to edit5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% Hints: get(hObject,'String') returns contents of edit5 as text
%        str2double(get(hObject,'String')) returns contents of edit5 as a double% --- Executes during object creation, after setting all properties.
function edit5_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))set(hObject,'BackgroundColor','white');
endfunction edit6_Callback(hObject, eventdata, handles)
% hObject    handle to edit6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% Hints: get(hObject,'String') returns contents of edit6 as text
%        str2double(get(hObject,'String')) returns contents of edit6 as a double% --- Executes during object creation, after setting all properties.
function edit6_CreateFcn(hObject, eventdata, handles)
% hObject    handle to edit6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    empty - handles not created until after all CreateFcns called% Hint: edit controls usually have a white background on Windows.
%       See ISPC and COMPUTER.
if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor'))set(hObject,'BackgroundColor','white');
end% --- Executes on button press in pushbutton1.
function pushbutton1_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% --- Executes on button press in pushbutton3.
function pushbutton3_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton3 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% --- Executes on button press in pushbutton5.
function pushbutton5_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton5 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% --- Executes on button press in pushbutton6.
function pushbutton6_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton6 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% --- Executes on button press in pushbutton7.
function pushbutton7_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton7 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% --------------------------------------------------------------------
function file_Callback(hObject, eventdata, handles)
% hObject    handle to file (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% --------------------------------------------------------------------
function open_Callback(hObject, eventdata, handles)
% hObject    handle to open (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
global im;
[filename,pathname]=uigetfile({'*.jpg';'*bmp';'*.gif'},'选择图片');
%合成图片路径+文件名
str=[pathname filename];
%读取图片
im=imread(str);
%使用第一个axes
axes(handles.axes0);
imshow(im);% --------------------------------------------------------------------
function save_Callback(hObject, eventdata, handles)
% hObject    handle to save (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)% --- Executes on button press in pushbutton8.
function pushbutton8_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton8 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
close(gcf) %关闭当前figure句柄% --- Executes on button press in pushbutton9.
function pushbutton9_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton9 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
global im
global I
global C
%%%%%%%%%%%%%%%%%%
I=im;
R=I(:,:,1);
G=I(:,:,2);
B=I(:,:,3);
K=rgb2hsv(I);
H=K(:,:,1);
S=K(:,:,2);
V=K(:,:,3);
[cenwhite,copywhite]=Kmeans(H,3);
cenwhite;
A=copywhite;
[m,n]=size(A);
for i=1:m for j=1:n if A(i,j)==cenwhite(2) A(i,j)=1; else A(i,j)=0; end end

三、运行结果

四、matlab版本及参考文献

1 matlab版本
2014a

2 参考文献
[1] 蔡利梅.MATLAB图像处理——理论、算法与实例分析[M].清华大学出版社，2020.
[2]杨丹,赵海滨,龙哲.MATLAB图像处理实例详解[M].清华大学出版社，2013.
[3]周品.MATLAB图像处理与图形用户界面设计[M].清华大学出版社，2013.
[4]刘成龙.精通MATLAB图像处理[M].清华大学出版社，2015.
[5]赵勇,方宗德,庞辉,王侃伟.基于量子粒子群优化算法的最小交叉熵多阈值图像分割[J].计算机应用研究. 2008,(04)

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