PacBio English 经典语句

2024-04-08 17:40:22

Another consideration is the relatively high cost of PacBio.

精选另一个考虑因素是PacBio的成本相对较高。

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引言来源论著2018IF 12.1Nature Communications

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Shorter PacBio reads fall into this category as well.

精选较短的PacBio读数也属于这一类。

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引言来源论著2019IF 11.5Nucleic ACIds Research

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We performed PacBio sequencing for yl to dissect the possible reason of the phenotype.

我们对yl进行PacBio测序以剖析表型的可能原因。

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引言来源论著2019IF 3.6BMC Genomics

Recently, PacBio throughput and cost per base have improved to the point that de novo plant genome assemblies using only PacBio are possible.

最近，PacBio的通量和每个碱基的成本已经提高到仅使用PacBio的从头植物基因组组装是可能的。

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引言来源论著2017IF 3.6BMC Genomics

As with PacBio, MinION read quality is low compared to short-read sequencing technologies.

与PacBio一样，与短读测序技术相比，MinION读取质量较低。

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引言来源论著2019IF 3.6BMC Genomics

For example, in 2016, ATCC 17978 was re-sequenced using PacBio.

例如，在2016年，使用PacBio对ATCC 17978进行了重新测序。

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引言来源论著2019IF 4.6Microbial Genomics

The specifications for PacBio P6-C4 chemistry indicate that PacBio reads have an N50 of 14 kb with a maximum length of 40 kb.

PacBio P6-C4 chemistry的规范表明PacBio读数的N50为14 kb，最大长度为40 kb。

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引言来源论著2015IF 3.6BMC Genomics

For example, phased diploid assemblies of two plant and one fungal genome were generated by hierarchical approaches using 100X to 140X PacBio and a human genome was assembled from 46X PacBio plus physical map data.

例如，使用100X至140X PacBio通过分级方法产生两个植物和一个真菌基因组的分阶段二倍体组装，并且从46X PacBio加上物理图数据组装人类基因组。

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引言来源论著2017IF 3.6BMC Genomics

PBHoney and SMRT-SV are designed specifically for PacBio reads while Sniffles supports PacBio and ONT reads.

PBHoney和SMRT-SV专为PacBio读取而设计，而Sniffles支持PacBio和ONT读取。

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引言来源论著2019IF 5.6Bioinformatics

Fortunately, PacBio sequencing and Illumina sequencing are highly complementary to each other.

幸运的是，PacBio测序和Illumina测序彼此高度互补。

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引言来源论著2020IF 3.6BMC Genomics

BLASR was initially designed for mapping PacBio reads to a reference genome.

BLASR最初被设计用于将PacBio读数映射到参考基因组。

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引言来源论著2019IF 3.6BMC Genomics

To take advantage of the PacBio long reads and reduce costs, we developed PacBio-LITS, a large-insert targeted capture-sequencing method.

为了利用PacBio长读取并降低成本，我们开发了PacBio-LITS，一种大插入靶向捕获测序方法.

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引言来源论著2015IF 3.6BMC Genomics

Because most of the errors in PacBio sequencing are random, PacBio reads can be corrected by alignment to other PacBio reads, given sufficient coverage redundancy.

因为PacBio测序中的大多数错误是随机的，所以在给定足够的覆盖冗余的情况下，PacBio读取可以通过与其他PacBio读取对齐来校正。

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引言来源论著2017IF 3.6BMC Genomics

PacBio RS also represents an alternative to cloning and Sanger sequencing for longer rRNA amplicons.

PacBio RS也代表了克隆和Sanger测序更长rRNA扩增子的替代方案。

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引言来源论著2018IF 3.1BMC Evolutionary Biology

More recently, both PacBio and the research group responsible for Celera have released versions of their software packages which self-corrects PacBio reads by aligning reads to each other and generating consensus sequences.

最近，PacBio和负责Celera的研究小组都发布了他们软件包的版本，这些软件包通过相互对齐读取并生成一致序列来自我纠正PacBio读取。

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引言来源论著2013IF 3.6BMC Genomics

DNA was sent to the Duke Center for Genomics and Computational Biology facility, where a SMRTBell™ long insert PacBio library (15–20Kb fragments) was prepared and then sequenced using a PacBio RSII system (P6-C4 Chemistry).

DNA被送到杜克基因组学和计算生物学中心™制备长插入PacBio文库 (15-20Kb片段)，然后使用PacBio RSII系统 (P6-C4化学) 测序。

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引言来源论著2017IF 3.6BMC Genomics

In order to make use of PacBio data without sacrificing throughput and read length, several recent studies applied a strategy termed ‘hybrid sequencing’ to integrate PacBio long reads with high-quality SGS short reads (e.g. Illumina data).

为了在不牺牲吞吐量和读取长度的情况下利用PacBio数据，最近的几项研究应用了一种称为 “混合测序” 的策略，将PacBio长读取与高质量SGS短读取 (例如Illumina数据) 集成在一起。

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引言来源论著2015IF 11.5Nucleic ACIds Research

The Illumina reads are used to generate an assembly graph that serves as a backbone for subsequent PacBio-based steps.

Illumina读取用于生成装配图，该图用作后续基于PacBio的步骤的主干。

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引言来源论著2018IF 5.6Bioinformatics

However, PacBio and MinION generate reads with sequencing errors rates as high as 15 and 35 % respectively.

然而，PacBio和MinION产生的读数的测序错误率分别高达15和35%。

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引言来源论著2015IF 3.6BMC Genomics

This style of sequencing in turn allows for the third distinguishing characteristic of the PacBio – the ability to call covalent base modifications.

这种测序方式又允许PacBio的第三个区别特征-调用共价碱基修饰的能力。

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引言来源论著2013IF 3.6BMC Genomics

The first approach, PBHoney-Spots, exploits the stochastic nature of the errors in PacBio reads.

第一种方法，PBHoney-Spots，利用PacBio读取错误的随机性质。

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引言来源论著2019IF 5.6Bioinformatics

Application of PacBio sequencing however requires a specialised laboratory with the necessary capital investment in equipment.

然而，PacBio测序的应用需要一个专业实验室，在设备上进行必要的资本投资。

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引言来源论著2017IF 3.6BMC Genomics

The new method extends Illumina reads into super-reads and then combines these with the PacBio data to create mega-reads, essentially converting each PacBio read into one or more very long, highly accurate reads.

新方法将Illumina读取扩展为超级读取，然后将这些与PacBio数据组合以创建超级读取，基本上将每个PacBio读取转换为一个或多个非常长、高度准确的读取。

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引言来源论著2017IF 11.1Genome Research

In addition, the number of CCS reads is much less than all the output of PacBio data, for example, only 37 906 CCS reads (≥4 passes) were yielded from one SMRT cell of the PacBio RS II platform.

此外，CCS读取的数量远远少于PacBio数据的所有输出，例如，仅从PacBio RS II平台的一个SMRT单元产生了37-906个CCS读取 (≥ 4次通过)。

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引言来源论著2020IF 4.6Microbial Genomics

To our knowledge this is the first report of a chloroplast genome sequenced using PacBio RS data.

据我们所知，这是使用PacBio RS数据测序叶绿体基因组的第一份报告。

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引言来源论著2013IF 3.6BMC Genomics

We first evaluate strategies for assembling PacBio reads, and how they perform with differing amounts of sequence coverage.

我们首先评估组装PacBio读数的策略，以及它们在不同的序列覆盖量下的表现。

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引言来源论著2016IF 11.5Nucleic ACIds Research

Single molecule, real‐time (SMRT) sequencing, employed by the PacBio sequencing platforms, enables amplification‐free sequencing.

PacBio测序平台采用的单分子实时 (SMRT) 测序能够实现无扩增测序。

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引言来源论著2018IF 4.1Human Mutation

In 2011, PacBio RS II was developed as the first commercially available third-generation sequencer and it was marketed to address this issue.

在2011，PacBio RS II被开发为第一个市售的第三代测序仪，并且其上市是为了解决这个问题。

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引言来源论著2018IF 4.0Dna Research

PacBio LRS produces reads of several thousand base pairs with uniform coverage across sequence contexts.

PacBio LRS产生数千个碱基对的读数，在序列背景下具有均匀的覆盖。

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引言来源病例报道2018IF 8.9Genetics in Medicine

In addition, both PacBio and ONT can directly detect DNA methylation, providing additional valuable information for epigenetics.

此外，PacBio和ONT都可以直接检测DNA甲基化，为表观遗传学提供额外的有价值的信息。

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引言来源论著2018IF 11.5Nucleic ACIds Research

Single molecule sequencing, like that offered by Pacific Biosciences (PacBio), meets these criteria with reads that are routinely tens of kilobases in length.

单分子测序，就像太平洋生物科学 (PacBio) 提供的那样，符合这些标准，读取的长度通常是几十千碱基。

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引言来源论著2016IF 11.5Nucleic ACIds Research

In addition, compared with PacBio®, the Nanopore® technology apparatus is affordable in most laboratories.

此外，与PacBio相比®,纳米孔®技术设备在大多数实验室是负担得起的。

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引言来源论著2019IF 5.9Journal of Experimental Botany

PacBio-LITS leverages the cost efficiency of targeted capture-sequencing as well as the mappability and scaffolding capabilities of long sequencing reads generated by the PacBio platform.

PacBio-LITS利用了靶向捕获测序的成本效率以及PacBio平台生成的长测序读取的可映射性和支架能力。

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引言来源论著2015IF 3.6BMC Genomics

PacBio long-reads were assembled and annotated into 738 contigs of 123 Mb containing 29,769 genes.

PacBio长读段被组装并注释为包含738个基因的123个mb的29,769个重叠群。

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引言来源论著2019IF 3.6BMC Genomics

The PacBio RS II system is capable of detecting DNA methylation through analysis of polymerase kinetics.

Pacbionrsii系统能够通过聚合酶动力学分析来检测DNA甲基化。

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引言来源论著2014IF 4.2Frontiers in Microbiology

BLASR is the first tool specifically designed for PacBio reads.

BLASR是第一个专门为PacBio读取设计的工具。

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引言来源论著2019IF 5.6Bioinformatics

A tutorial, developed by the PacBio team recommends modified sets of parameters for the alignment of PacBio reads with STAR and GMAP, based on in-house testing.

PacBio团队开发的一个教程基于内部测试，推荐了PacBio读取与STAR和GMAP对齐的修改参数集。

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引言来源论著2018IF 5.6Bioinformatics

The majority of PacBio reads (referred to as subreads) are too long to generate CCS reads, but they are very informative.

大多数PacBio读取 (称为子读取) 太长，无法生成CCS读取，但它们非常有用。

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引言来源论著2015IF 11.5Nucleic ACIds Research

PacBio reads were used directly in Organelle_PBA without any extra processing.

PacBio读取直接在Organelle_PBA中使用，无需任何额外处理。

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引言来源论著2017IF 3.6BMC Genomics

Here we describe the use of Pacbio sequencing to characterize the fungal community associated with Castanopsis carlesii deadwood.

在这里，我们描述了使用Pacbio测序来表征与苦cast相关的真菌群落。

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引言来源论著2019IF 4.2Frontiers in Microbiolog

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