BLAST Database error: No alias or index file found for protein database

在运行PROVEAN软件时，指定的nr数据库路径是：/data/software/blastdb/nr，给出的报错是BLAST Database error: No alias or index file found for protein database [/data/software/blastdb/nr/nr] in search path

# nr数据库下的文件
(base) [user@localhost examples]$ ll /data/software/blastdb/nr/
total 559692336
-rw-rw-rw- 1 root root 236883819668 Mar  3 04:14 nr
-rw-rw-r-- 1 user user    527272381 Mar  3 21:44 prot_fa.00.phr
-rw-rw-r-- 1 user user     20869088 Mar  3 21:44 prot_fa.00.pin
-rw-rw-r-- 1 user user     10434532 Mar  3 21:44 prot_fa.00.pog
-rw-rw-r-- 1 user user    999999663 Mar  3 21:44 prot_fa.00.psq
-rw-rw-r-- 1 user user    526521210 Mar  3 21:45 prot_fa.01.phr
-rw-rw-r-- 1 user user     20867784 Mar  3 21:45 prot_fa.01.pin
-rw-rw-r-- 1 user user     10433880 Mar  3 21:45 prot_fa.01.pog
-rw-rw-r-- 1 user user    999999510 Mar  3 21:45 prot_fa.01.psq
-rw-rw-r-- 1 user user    526400446 Mar  3 21:46 prot_fa.02.phr
-rw-rw-r-- 1 user user     20891984 Mar  3 21:46 prot_fa.02.pin
-rw-rw-r-- 1 user user     10445980 Mar  3 21:46 prot_fa.02.pog
-rw-rw-r-- 1 user user    999999932 Mar  3 21:46 prot_fa.02.psq
-rw-rw-r-- 1 user user    526061308 Mar  3 21:47 prot_fa.03.phr
-rw-rw-r-- 1 user user     20859688 Mar  3 21:47 prot_fa.03.pin

把provean.sh中BLAST_DB路径改为/data/software/blastdb/nr/prot_fa即可

blastp命令运行

blastp -query P04637.fasta -db /data/software/pdb/PDB -outfmt 6 -num_threads 12

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