注意：若在gridview内对radiobutton用组名groupname同名方法行不同，若加上GroupName="skytest"

查看静态页面源代码便知道：两个name值的组名都不同。

下面结合js实现单选.

呈现页：

<asp:GridView ID="GridView1" Width="960px" EmptyDataText="暂无标书可操作"
            DataKeyNames="ID" runat="server" onrowdatabound="GridView1_RowDataBound"
            >
            <Columns>
                <asp:TemplateField HeaderText="选择">
                    <ItemTemplate>
                        <asp:RadioButton ID="RadioButton1" runat="server"    />
                    </ItemTemplate>
                </asp:TemplateField>
                <asp:BoundField DataField="ID" HeaderText="标书编号" />

</Columns>
  </asp:GridView>

代码页：

protected void GridView1_RowDataBound(object sender, GridViewRowEventArgs e)
        {
            if (e.Row.RowType == DataControlRowType.DataRow)
            {
                RadioButton rb = (RadioButton)e.Row.FindControl("RadioButton1");
                if (rb != null)
                {
                    rb.Attributes.Add("onclick", "single(this)");  //single(obj)为js函数
                }
            }

}

需要添加的javascript函数：

<script type="text/javascript">
    var last=null;
    function single(obj)
    {
      if(last==null)    //第一次选择RadioButton时赋id值给last
      {
         last=obj.id; 
      }
      else            //第一次以后的每一次都在这运行，把上此的RadioButton.Checked=false,记下此次的obj.name
      {
        var lo=document.getElementByIdx_x(last);
        lo.checked=false;
        last=obj.name;
      }
      obj.checked="checked";   //添加checked属性，以便在上边赋值为false
    }
    </script>